Work package number |
4 |
Lead Beneficiary |
UTH |
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Work package title |
Assessment of the impact of MNP and combined effects with other stressors on soil biodiversity |
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Participant number |
1 |
3 |
4 |
6 |
9 |
10 |
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Short name of participant |
WU |
FIBL-CH |
UTH |
UCSC |
FUB |
INRAE |
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Person/months per participant |
14 |
2 |
33 |
13 |
20 |
16.5 |
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Start month |
9 |
End month |
60 |
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Objectives of the work package: The main objective of WP4, fully aligned with SO4 and SO5, will be to assess the effects of MNP (alone and in combination with other soil stressors) on the function and diversity of the soil biota. Specific experiments will be performed (T4.1) so that analysis can be run at different scales and supported by WP5 and WP6 (SO2). WP4 specific objectives are: (a) to determine the effects of MNP of different, size chemistry and origin on the function (T4.2) and diversity (T4.3) of the soil microbial community, including endophytes and rhizosphere microorganisms (b) to determine the effects of MNP of different chemistry, size and origin on the diversity and functions of soil micro- and macrofauna (T4.4) (c) to define effects on food web interactions and soil network configuration with links to function (T4.5) and (d) to evaluate the interactive effects of MP linked with their additives and other pollutants, often associated with MNP in agricultural soils (i.e. pesticides, veterinary drugs, additives), on the soil microbiota and soil fauna (all tasks). Results will be integrated, with data from WP5 and WP6, into an overall sustainability analysis (WP7) and stored as described in WP2. |
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Task 4.1 Implementation of multi-scale experimental plan Task leader: UTH| Participant(s): WU, FIBL-CH, UCSC, FUB, INRAE
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Task 4.2 Assessment of the impact of MNP and other stressors on functional attributes of soil microorganisms Task leader: FUB | Participant(s): INRAE,UTH,UCSC,FiBL-CH| We will assess effects on key functional microbial attributes including:
The functional endpoints to be measured at each experimental scale as defined in T4.1 are:
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Task 4.3 Assessment of the impact of MNP and other stressors on the diversity of soil microorganisms Task leader: INRAE | Participant(s): FUB, UTH, UCSC, WU| The methodology to determine the quantity and the quality (e.g. polyethylene, polypropylene, polystyrene, polyurethanes, synthetic oxo- and photo-degradable plastics, and biodegradable plastics) of plastic residues in soil will be standardized and included in the monitoring plan (T2.4). We will determine effects on soil microbial diversity via amplicon sequencing targeting:
(b) AMF intraradical diversity (FUB, UTH) (c) AOM diversity (i.e. AOA, AOB, commamox bacteria) (UTH).
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Task 4.4 Assessment of the impact of MNP and other stressors on the soil fauna Task leader: UCSC | Participant(s): WU,UCSC| We will determine in pot and field experiments the MNP effects on micro-, meso and macro-fauna targeting:
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Task 4.5: Effects of MNP and other stressors on soil food web interactions and network configuration Task leader: WU | Participant(s): WU| In this task, biodiversity data obtained in all tasks in WP4 will be merged to test whether MNP: (a) have increasing effect sizes on larger soil animals than on smaller microorganisms. We will comparatively assess differences in community metrics such as diversity and community composition between different groups of organisms. We will use different statistical approaches, to investigate potential differences of treatments on soil biodiversity groups; (b) change the configuration of soil biodiversity networks. We will combine all data obtained to construct co-occurrence networks across trophic levels and calculate different metrics on the network scale; (c) affect interactions between different (trophic) groups of soil organisms. We will use structural equation modelling to examine the magnitude of causal effects of MNP on trophic food-web interactions. Targeted multi-trophic in vitro tests (WU), including bacteria, fungi, protists, nematodes and larger soil animals in the presence of MNP will validate data observed in (a) and (b) and identify routes |
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Deliverable 4.1.: Protocol for multi-scale experiments [M11, UTH, R, PU] Deliverable 4.2: Guidelines on the assessment of the toxicity of MNP and other stressors on soil microbial functioning [M46, FUB, R, PU] Deliverable 4.3: Guidelines on the assessment of the toxicity of MNP and other stressors on soil microbial diversity [M49, INRAE, R, PU] Deliverable 4.4: List of soil biota indicators affected by MNP and other stressors [M52, WU, R, PU] Deliverable 4.5: Guidelines on the assessment of the toxicity of MNP and other stressors on soil fauna [M56, UCSC, R, PU] Deliverable 4.6: Guidelines for assessing effects of MNP and other stressors on the entire soil food web [M60, WU, R, PU] |